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A/M: (iastrointestinal stromal tumors (GISTS) represent an heterogeneous group of neoplasms with usually a non predictable biologic behavior The criteria of malignancy of these tumors (GISTS) include tumor size, number of mitoses per power fieId, presence of atypia and necrosis mucosa inldtration, peiomorpbisrn and cellularity GISTS are expressing the kit receptor (c-k~t), a tyrosine kinase receptor w'inch is coded by the c-kit protooncogene, in addition, they usually express CD 117 and CD34 antigens while, in rare cases they may be also positive tur SMA (smooth muscle antigen) desmin/HHF-35 (lean musck antigen), 3100 prolein (neural tissue) molecules or they may be negative to all the above antigens.The aim of the study was the determination of the biologic behavior of GISTS with immunohistochemistry MATERIA1.S METHODS: Using the stmpravidin-viotin method (Dako) 52 GISTS (biopsy specimens or surgically removed segments) diagnosed during the last decade in our hospital were immunohistochemmically studied tot the antigen c-kit (CD117), CD34, S},s desmin and S-100 ant gens In addinon p53 and ki-67 expression was studied in order to deternrine tumor biological behavmr Sta isrical analysis was pedbrmed using the SPPS working package RESULTS: From h e total number ot mesenchymal tunmrs studied based on na:>rphology and ir.arnunohistochemist U, 474% of GISTS were benign and 526% were malignant There was a statistically significant cormlatinn of GISTS malignant behawor veith p53 arid ki-67 in~munostainmg ( p = 0 0 0 1 6 and p = 0 0 0 3 respectively). No statistically signtficam correlation of ck i t (CDl17) antigen, CD68, CDIO, CD99 and SMA with p53 and ki-67 was {bund CONCLUSIONS: GISTS represent tumors with a more less undefined biological beha,nor lmmunohistochemist U studies in these tumors mainly using p53 and ki-67 immcmostaining can assist diagnosis and prognosis The p53 and k>57 immunohistochemist~" has a high potential ofdetemfination of malignant or benign behavior and prognosis of the~ rare gastrointestinal tumors |
