Simultaneous analysis of ten components in patch preparation of Wan-Yin-Gau by high performance liquid chromatography
| Autor: | Tian Shung Wu, Chia Chi Chen, Horng Liang Lay, Shiow Chyn Huang, Thau Ming Cham |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Pharmacology
Coptisine 0303 health sciences Chromatography 030309 nutrition & dietetics Elution 010401 analytical chemistry Paeoniflorin 01 natural sciences High-performance liquid chromatography 0104 chemical sciences 03 medical and health sciences chemistry.chemical_compound chemistry Radix Emodin Phosphoric acid Baicalin Food Science |
| Zdroj: | Journal of Food and Drug Analysis. 13 |
| ISSN: | 2224-6614 |
| Popis: | A facile HPLC method for the resolution and quantitative measurement of seven marker substances, the active ingredients in patch preparation of Rui-Yun-Gau, was established under the gradient elution in the reversed-phase mode. These marker substances included berberine (Coptidis Rhizoma and Phellodendri Cortex), and coptisine (Coptidis Rhizoma), emodin, and sennoside A (Rhei Rhizoma), harpagoside (Scrophulariae Radix), baicalin, and baicalein (Scutellariae Radix). The ingredients in the water-based and oil-based patches of the formula from different manufactures were also analyzed for quality evaluation. Extracted samples were analyzed with a reversed-phase column (Inertsil 5 ODS-2, 4.6 i.d. ×250 mm) at 30℃, eluted with a mixture of 10% acetonitrile and 60% acetonitrile aqueous solution (each adjusted to pH 2.8 with phosphoric acid) in gradient manner at a flow-rate of 1.0 mL/min, and detected at 240 nm. Relative coefficients of variations of intra- and inter-day analysis were less than 5%. All the recoveries were 90.65~112.22%, therefore this method could be applied for the simultaneous determination of seven marker substances in ”Rui-Yun-Gau”. |
| Databáze: | OpenAIRE |
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