Prostaglandin receptors IP and EP mediating regulation of tumor necrosis factor-alpha and interleukin-10 production
| Autor: | Iku Utsunomiya, Koichi Yuki, Shiho Shinomiya, Shuichi Ohuchida, Hiroaki Naraba, Sachiko Oh-ishi, Akinori Ueno, Fumitaka Ushikubi, Takayuki Maruyama, Atsushi Ichikawa, Shuh Narumiya, Yukihiko Sugimoto |
|---|---|
| Rok vydání: | 2002 |
| Předmět: |
Agonist
medicine.medical_specialty medicine.drug_class medicine.medical_treatment Prostaglandin E2 receptor Prostaglandin General Medicine chemistry.chemical_compound Interleukin 10 Cytokine Endocrinology chemistry Internal medicine medicine lipids (amino acids peptides and proteins) Tumor necrosis factor alpha Receptor Prostaglandin E |
| Zdroj: | International Congress Series. 1233:479-484 |
| ISSN: | 0531-5131 |
| Popis: | We examined the roles of prostaglandin receptors regulating tumor necrosis factor-alpha (TNFα) and IL-10 production by zymosan-stimulated murine peritoneal macrophages. During the stimulation with zymosan for 5 h, the presence of PGE2 or carbacyclin suppressed the TNFα production, whereas it increased that of IL-10; and indomethacin caused the reverse effects, suggesting that endogenous prostaglandins may have regulatory effects on the cytokine production. Among the EP subtype-selective synthetic agonists used, EP2 and EP4 agonists caused down-regulation of the TNFα production, but up-regulation of the IL-10 production; whereas EP1 and EP3 agonists showed no effect. Macrophages harvested from IP, EP2 or EP4 receptor-deficient mice lacked the up- and down-regulatory effects on the cytokine production by each corresponding agonist. These effects of prostaglandins agreed well with the mRNA expression of TNFα and IL-10 when mRNA levels were examined by the RT-PCR method. Thus, the results suggest that endogenous PGI2 and PGE2 may exert their anti-inflammatory influence on the cytokine production through IP, EP2, and EP4 receptors. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect