Analysis ofParacoccidioideslutziimitochondria: a proteomic approach
| Autor: | Luciana Casaletti, Clayton Luiz Borges, Alexandre Mello Bailão, Sônia Nair Báo, C. M. A. Soares, Lucas Nojosa Oliveira, Patrícia de Sousa Lima |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
030102 biochemistry & molecular biology Autophagy Cell Glyoxylate cycle Bioengineering Oxidative phosphorylation Mitochondrion Biology Applied Microbiology and Biotechnology Biochemistry 03 medical and health sciences Metabolic pathway 030104 developmental biology medicine.anatomical_structure Organelle Genetics medicine Dimorphic fungus Biotechnology |
| Zdroj: | Yeast. 34:179-188 |
| ISSN: | 0749-503X |
| DOI: | 10.1002/yea.3225 |
| Popis: | The genus Paracoccidioides is composed of thermal dimorphic fungi, causative agents of paracoccidioidomycosis, one of the most frequent systemic mycoses in Latin America. Mitochondria have sophisticated machinery for ATP production, which involves metabolic pathways such as citric acid and glyoxylate cycles, electron transport chain and oxidative phosphorylation. In addition, this organelle performs a variety of functions in the cell, working as an exceptional metabolic signaling center that contributes to cellular stress responses, as autophagy and apoptosis in eukaryotic organisms. The aim of this work was to perform a descriptive proteomic analysis of mitochondria in Paracoccidioides lutzii, yeast cells. After mitochondria fractionation, samples enriched in mitochondrial proteins were digested with trypsin and analyzed using a NanoUPLC-MSE system (Waters Corporation, Manchester, UK). Ours results revealed that the established protocol for purification of mitochondria was very effective for P. lutzii, and 298 proteins were identified as primarily mitochondrial, in our analysis. To our knowledge, this is the first compilation of mitochondrial proteins from P. lutzii, to date. This article is protected by copyright. All rights reserved. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text