| Popis: |
New saponin components, Sg-6 saponins, has been recently reported from the seeds of wild soybean (Glycine soja) which may have specific health benefits. To evaluate the possible health benefits, a large amount of Sg-6 saponins is needed, but general group A acetyl saponins and new Sg-6 saponins are eluted in overlapping peaks by ordinal preparative high-performance liquid chromatography (HPLC) and/or open column methods. A new method is proposed in this report. This method includes 1) deacetylation of group A acetyl saponins in alkali condition with KOH, 2) precipitation of Sg-6 saponins in acid condition with HCl, 3) recovery of Sg-6 saponins with aqueous methanol from the precipitate, and 4) elution of Sg-6 saponins by preparative reverse-phase open column. With this method, from 450 g of wild soybean whole seed powder, about 1 g of Sg-6 saponins (mixture of 6 components) were clearly separated from other saponins with 61% recovery. |
