The development of three SNP assays for forensic casework

Autor:	Burkhard Madea, T. Senge, Anke Junge
Rok vydání:	2006
Předmět:	Genetics education.field_of_study Chromosome (genetic algorithm) Chromosome 3 Genotype Population SNP Single-nucleotide polymorphism General Medicine Biology education Molecular Inversion Probe SNP genotyping
Zdroj:	International Congress Series. 1288:46-48
ISSN:	0531-5131
DOI:	10.1016/j.ics.2005.09.019
Popis:	The present work introduces three novel validated SNP assays and describe their applicability in forensic casework. For this study three unlinked and non-coding SNPs named TSC0582423 (Chromosome 2), TSC0171847 (Chromosome 1) and TSC0741184 (Chromosome 3) with a balanced allele distribution were selected from the database of the SNP consortium. SNP detection was based on the 5′ nuclease system from Applied Biosystems. Before the use of this SNP-detection system in the laboratory routine, validation studies must be performed including determination of SNP genotypes by sequencing, sensitivity/reproducibility studies and population studies. Tests with artificial stains and with degraded DNA samples were also performed.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::3762f288dc18bec46f7ad7cafc84ec5e https://doi.org/10.1016/j.ics.2005.09.019 Full Text from ScienceDirect