Decreased nitric oxide-mediated natural killer cell activation in chronic fatigue syndrome
| Autor: | Yoko Horikawa, Yuji Matsuzawa, Jun Ishikawa, Yoshio Kanayama, Megumu Ogawa, Tetsuo Nishiura, Masafumi Yoshimura, Hirohisa Nakao, Yoshiaki Tomiyama, Itaru Matsumura, Yu Okajima, Hitoshi Yoshida, Yuzuru Kanakura |
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| Rok vydání: | 1998 |
| Předmět: |
medicine.medical_specialty
Clinical Biochemistry General Medicine Biology medicine.disease Biochemistry Peripheral blood mononuclear cell Nitric oxide Natural killer cell Pathogenesis chemistry.chemical_compound Endocrinology medicine.anatomical_structure chemistry Internal medicine Immunology medicine Chronic fatigue syndrome S-Nitroso-N-acetylpenicillamine Cell activation Natural killer cell activation |
| Zdroj: | European Journal of Clinical Investigation. 28:937-943 |
| ISSN: | 0014-2972 |
| DOI: | 10.1046/j.1365-2362.1998.00373.x |
| Popis: | Background L-Arginine ( L-Arg), one of the essential amino acids, has been reported to have an immunomodulatory effect. The precise mechanism of the L-Arg-induced natural killer (NK) cell activation remains unresolved ,and the effect of L-Arg on NK cells in chronic fatigue syndrome (CFS) patients has not been estimated. Methods NK cell function was evaluated in 20 subjects with CFS and compared with that in 21 healthy individuals. Results In healthy control subjects, NK activity was significantly increased after treatment with L-Arg, an NK function enhancer, for 24 h, whereas the same treatment failed to enhance NK activity in the CFS patients. We thus focused on L-Arg metabolism, which involves nitric oxide (NO) production through NO synthase (NOS). The expression of inducible NO synthase (iNOS) transcripts in peripheral blood mononuclear cells was not significantly different between healthy control subjects and CFS patients. The L-Arg-mediated NK cell activation was abolished by addition of NG-monomethyl- L-arginine, an inhibitor for iNOS. Furthermore, incubation with S-nitroso-N-acetyl-penicillamine, an NO donor, stimulated NK activity in healthy control subjects but not in CFS patients. Conclusion These results demonstrate that the L-Arg-induced activation of NK activity is mediated by NO and that a possible dysfunction exists in the NO-mediated NK cell activation in CFS patients. |
| Databáze: | OpenAIRE |
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