Protein Content of Circulating Nucleoprotein Complexes
| Autor: | Svetlana N. Tamkovich, Danil S. Serdukov, Pavel P. Laktionov, Yuri P. Tsentalovich, Natalia A. Kirushina, O. S. Tutanov, Vsevolod A. Tkachuk, Maxim S. Belenikin, Vladimir E. Voytsitskiy, Anatoliy G. Shlikht |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
030102 biochemistry & molecular biology biology Deoxyribonucleoprotein Chemistry DNA-binding protein Nucleoprotein 03 medical and health sciences chemistry.chemical_compound 030104 developmental biology Biochemistry Affinity chromatography Polyclonal antibodies Blood plasma biology.protein Antibody DNA |
| Zdroj: | Advances in Experimental Medicine and Biology ISBN: 9783319420424 |
| DOI: | 10.1007/978-3-319-42044-8_26 |
| Popis: | In the current study we have investigated the protein content of blood plasma deoxyribonucleoprotein complexes. The complexes were isolated using affinity chromatography with immobilized polyclonal anti-histone antibodies. Proteins were separated by SDS PAAGE and identified by MALDI-TOF mass-spectrometry. 111 and 56 proteins (excluding histones), respectively, were identified with a good score in deoxyribonucleoprotein complexes of healthy females and breast cancer patients. However, only four of these proteins were found in 30 % of all samples. Fourteen proteins previously described as tumor specific proteins were found in cancer patients whereas not one of them was found in healthy individuals. The data obtained demonstrate the involvement of different cellular and extracellular proteins in circulating cell-free DNA. |
| Databáze: | OpenAIRE |
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