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In a chronic adaption feeding regimen, both D-fructose and vanadate independently altered glucose oxidation and lipogenesis in adipose tissue of rats. This study investigated the acute feeding effects of vanadate and D-fructose on these parameters in isolated rat adipocytes. In a 3-d fasting/refeeding regimen, male Wistar rats (10/group), weighing 150–200 g, were fed a diet containing either glucose (control) or fructose at 27% (ww) with sodium orthovanadate at 0, 25 (vanadate-1), and 50 (vanadate-2) mg/kg of diet. Neither body weight gain nor food efficiency differed statistically between treatment groups, but lower food intake in vanadate-treated animals suggests a compensatory effect of food efficiency in maintaining body weight gain at the same level as in other groups. Basal glucose oxidation to CO 2 was enhanced in a dose-related fashion in glucose-vanadate groups, but a significant interaction between fructose and vanadate led to a reduction in vanadate-induced basal glucose oxidation. The reduction in basal and insulin-stimulated glucose conversion to lipid in adipocytes and in hepatic glucose 6-phosphate dehydrogenase and malic enzyme activities by vanadate was not altered by fructose. These results suggest that, under acute feeding, fructose alters the insulin-mimetic aspect of vanadate involving glucose conversion to CO 2 in isolated adipocytes. |
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