| Autor: |
Fourel Geneviève, Corre Guillaume, Sudharshan Ravi, Gunawan Rudiyanto, Parmentier Romuald, Moussy Alice, Paldi Andras, Papili Gao Nan, Chantalat Sophie, Racine Laëtitia, Stockholm Daniel, Deleuze Jean-François |
| Rok vydání: |
2020 |
| Předmět: |
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| Popis: |
SummaryWhen human cord blood derived CD34+ cells are induced to differentiate in vitro, they undergo rapid and dynamic morphological and molecular transformations that are critical for fate commitment. Using ATAC-seq and single-cell RNA sequencing, we detected two phases in this process. In the first phase, we observed a rapid and global chromatin opening that makes most of the gene promoters in the genome accessible, followed by widespread upregulation of gene transcription and a concomitant increase in the cell-to-cell variability of gene expression. The second phase is marked by a slow chromatin closure and a subsequent overall downregulation of gene transcription and emergence of coherent expression profiles corresponding to distinct cell subpopulations. These observations are consistent with a model based on the spontaneous probabilistic organization of the cellular process of fate commitment. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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