Molecular, morphological and pathogenic characterization of Clavibacter michiganensis subsp. sepedonicus strains of different geographic origins in Georgia
| Autor: | Shorena Kharadze, Dali Gaganidze, Tinatin Sadunishvili, G. Kvesitadze, Edward Arseniuk, Nanuli V. Amashukeli, Neli Sturua, Lia L. Amiranashvili, Agnieszka Węgierek-Maciejewska |
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| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
0301 basic medicine Veterinary medicine Genetic diversity fungi food and beverages Virulence Plant Science Horticulture Biology Cell morphology biology.organism_classification 01 natural sciences 03 medical and health sciences Restriction enzyme Variable number tandem repeat 030104 developmental biology health services administration Bioassay Agronomy and Crop Science Clavibacter michiganensis Pathogen health care economics and organizations 010606 plant biology & botany |
| Zdroj: | European Journal of Plant Pathology. 158:195-209 |
| ISSN: | 1573-8469 0929-1873 |
| DOI: | 10.1007/s10658-020-02066-x |
| Popis: | Potato ring rot and its causative pathogen Clavibacter michiganensis subsp. sepedonicus (Cms) have been studied in Georgia for the first time. The samples were collected in the fields and potato storage facilities in various potato producing regions of Georgia. Potato tubers were also collected at agrarian markets and the border checkpoints, from which potatoes are imported from neighboring countries. In 124 potato samples the detection of Cms was performed using specific PCR analysis and bioassay test. A total of 20 Cms isolates were purified and identified by subspecies-specific PSA-1 and PSA-R primers set. The isolates were characterized according to cell morphology, cultural-biochemical properties and virulence. The study of genetic diversity using molecular typing methods of PCR melting profile with different restriction enzymes and variable number tandem repeats PCR revealed significant differences between Cms isolates, which is not related to their geographical origin. |
| Databáze: | OpenAIRE |
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