| Autor: |
Virgil B. Elings, Magdalena Bezanilla, Jay C. Groppe, Paul V. C. Hough, Helen G. Hansma, G. Gurley, Robert L. Sinsheimer, Paul K. Hansma, Iris A. Mastrangelo, Thomas C. Bruice |
| Rok vydání: |
1993 |
| Předmět: |
|
| Zdroj: |
Scanning. 15:296-299 |
| ISSN: |
0161-0457 |
| DOI: |
10.1002/sca.4950150509 |
| Popis: |
Three advances involving DNA in atomic force microscopy (AFM) are reported here. First a HEPES-Mg buffer has been used that improves the spreading of DNA and provides good DNA coverage with as little as 200-500 picograms per sample. Second, the new "tapping" mode has been used to improve the ease and resolution of AFM-imaging of DNA in air. Finally, AFM images are presented of single-stranded phi X-174 virion DNA with the gene 32 single-stranded binding protein. A summary of the current state of the field and of the methods for preparing and imaging DNA in the AFM is also presented. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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