Signal transduction mechanisms in substance P–mediated ciliostimulation
Autor: | Thomas V. McCaffrey, Rodney J. Schlosser, Bin Yang, Judith M. Czaja |
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Rok vydání: | 1995 |
Předmět: |
medicine.medical_specialty
Arginine business.industry Prostaglandin Neuropeptide Substance P Nitric oxide chemistry.chemical_compound Endocrinology Otorhinolaryngology chemistry Mechanism of action Internal medicine Second messenger system medicine Surgery medicine.symptom Respiratory system business |
Zdroj: | Otolaryngology - Head and Neck Surgery. 113:582-588 |
ISSN: | 0194-5998 |
DOI: | 10.1016/s0194-5998(95)70049-8 |
Popis: | Substance P is a neuropeptide released by afferent neurons in the respiratory tract during inflammatory reactions. It produces effects on blood vessels, bronchial smooth muscle, nasal glands, and respiratory cilia. We studied the in vitro effect of substance P on the ciliary beat frequency of human adenoid explants and its mechanism of action. Substance P was added to cultured adenoid at concentrations of 10 -10 , 10 -8 , 10 -6 , and 10 -4 mol/L. Ciliary beat frequency was determined with phase-contrast microscopy and microphotometry. Substance P increased ciliary beat frequency a maximum of 11.9%±3.8% ( p -6 mol/L) significantly blocked the ciliostimulatory effects of SP ( p L -arginine analogs, N G -nitro- L -arginine methyl ester and N G -monomethyl- L -arginine, inhibit nitric oxide synthesis from L -arginine. L -Arginine analogs (10 -4 to 10 -2 mol/L) inhibited the effect of substance P ( p L -arginine, demonstrating that nitric oxide production is a required step in substance P-induced ciliostimulation. Substance P stimulates ciliary activity in human nasal mucosa as a result of secondary production and release of endogenous prostaglandins and nitric oxide. It is likely that inflammatory disease processes that stimulate release of substance P and subsequent prostaglandin and nitric oxide production modify mucociliary transport. Pharmacologic modification of substance P and its second messengers may eventually permit regulation of this important defense mechanism and control of neurogenic inflammation.(OTOLARYNGOL HEAD NECK SURG 1995;113:582-8.) |
Databáze: | OpenAIRE |
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