Entrapment of microbial cells and organelles with hydrophilic urethane prepolymers

Autor:	Susumu Kawamoto, Atsuo Tanaka, Ing-Nyol Jin, Saburo Fukui
Rok vydání:	1979
Předmět:	chemistry.chemical_classification biology Chemistry General Medicine Peroxisome Applied Microbiology and Biotechnology Microbiology Yeast Alcohol oxidase Enzyme Biochemistry Catalase Organelle biology.protein Microbody Prepolymer Biotechnology
Zdroj:	European Journal of Applied Microbiology and Biotechnology. 7:351-354
ISSN:	1432-0614 0171-1741
DOI:	10.1007/bf00499849
Popis:	Microbial cells and cellular organelles were immobilized by mixing aqueous suspensions of the biocatalysts with water-miscible urethane prepolymers. Thus immobilized preparations of acetone-dried cells of Arthrobacter simplex and thawed cells of Nocardia rhodocrous showed appreciable {ie351-1} activities in the transformation of hydrocortisone into prednisolone and 4-androstene-3,17-dione to androst-1,4-diene-3,17-dione, respectively. The activities of catalase and alcohol oxidase were observed in the immobilized peroxisomes (microbodies) of a methanol-grown yeast Kloeckera sp. No. 2201. Yeast mitochondria entrapped with the prepolymer showed adenylate kinase activity. These results indicate the usefulness of the urethane prepolymers as convenient materials for entrapment of not only enzymes, but also organelles and microbial cells.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::3718f7bc0e6bc4fd4d75dca8dbf2de1f https://doi.org/10.1007/bf00499849 Zobrazit plný text záznamu Full text from SpringerLink