Isolation and characterization of 12 polymorphic microsatellite markers from ladyfish (Elops saurus Linnaeus)
| Autor: | Shi-Chao Xing, Changwei Shao, Zhenxia Sha, Song-Lin Chen, Xiaolin Liao, Yongsheng Tian |
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| Rok vydání: | 2009 |
| Předmět: |
Genetics
Genetic diversity education.field_of_study Linkage disequilibrium biology Population Locus (genetics) biology.organism_classification Loss of heterozygosity Evolutionary biology Elops saurus Polymorphic Microsatellite Marker Microsatellite education Ecology Evolution Behavior and Systematics |
| Zdroj: | Conservation Genetics. 10:1799-1801 |
| ISSN: | 1572-9737 1566-0621 |
| DOI: | 10.1007/s10592-009-9819-y |
| Popis: | Ladyfish (Elops saurus Linnaeus) is an economically important marine fish species. 76 microsatellite loci were isolated from an enriched genomic library of Elops saurus. Twelve of these markers were polymorphic in a test population with alleles per locus ranging from three to nine. The number of observed, expected heterozygosity and polymorphism information content (PIC) per locus in 20 individuals ranged from 0.2000 to 1.0000, 0.1897-0.8846, 0.1769-0.8476, respectively. One markers significantly deviated from Hardy-Weinberg equilibrium after Bonferroni correction analysis and there was no significant linkage disequilibrium found between pairs of markers. As a result, 12 microsatellite markers probably should provide sufficient level of genetic diversity to investigate the fine-scale population structure, stock management and enhancement, genetic linkage map construction and molecular marker-assisted breeding in Elops saurus Linnaeus. |
| Databáze: | OpenAIRE |
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