| Autor: |
Daniel F. Simão, Luis F.L. Reis, Vanderlei Rodrigues, Dirce Maria Carraro, Marco Antônio Zago, Ismael Dale Cotrim Guerreiro da Silva, Rui M. B. Maciel, Heloisa Zalcberg, Alex F. Carvalho, Maria Lucia C. Corrêa, Daniela C. Miranda, Paula Rahal, Ari J. S. Ferreira, E. C. Miracca, Silvia Regina Rogatto, Ana L. T. O. Nascimento, Simone Cristina Sant'anna, Andrew J. G. Simpson, Nancy da Rós, Wilson A. Silva, Maria E. R. de Camargo, Christine Hackel, Gilberto K. Furuzawa, Fernando Lopes Alberto, Gustavo H. Goldman, Vanessa Bombonato, Elida B. Ojopi, Aline Maria Da Silva, Cláudia Aparecida Rainho, Angela Maria de Assis, Maria de Fátima Sonati, Maria Aparecida Nagai, Rosana F. R. Corrêa, Hamza El-Dorry, Maria Luísa Paço-Larson, Sergio Verjovski-Almeida, Nádia Aparecida Bérgamo, Fernando Augusto Soares, Mari Cleide Sogayar, Valeria Valente, Maria Inês de Moura Campos Pardini, Aline C. Prevedel, Arthur Gruber, Analy Salles de Azevedo Melo, Anamaria A. Camargo, Mozart Marins, Sandro Roberto Valentini, Janete M. Cerutti, Mariana Lopes dos Santos, Marina P. Nobrega, Francisco G. Nobrega, Eloiza H. Tajara, M. E. J. Amaral, Edna Teruko Kimura, Italo A. Migotto, Marcelo Lima Ribeiro, Daniel Giannella-Neto, Josane F. Sousa, M B Melo, Paula de Oliveira Montandon Hokama, José Rodrigo C. Pandolfi, Ricardo R. Brentani, Diana N. Nunes, Renata de Azevedo Canevari, Fernando Tsukumo, Liliane A. T. Arnaldi, Renata G. de Sá, Ivy Aneas, Elizabeth A. L. Martins, Mario H. Bengtson, Elza Kimura, Emmanuel Dias-Neto, Eduardo M. Reis, Daniella Stecconi, Suely Kazue Nagahashi Marie, Paromita Majumder, Rosana A Silveira, Carlos Alberto Mestriner, Enilza Maria Espreáfico, M. C. R. Costa, João Bosco Pesquero, Luciana C. C. Leite, José Eduardo Krieger, Sandro J. de Souza, Helena B. Samaia, Gonçalo A.G. Pereira, Helaine Carrer, Magaly M. Sales, Paulo L. Ho, Everaldo dos Reis Marques, Fernando Ferreira Costa, Ricardo G. Correa, Neusa Pereira da Silva, Angelita Habr-Gama, Luciana Gilbert Pessoa, Eloisa de Sá Moreira, Tsieko Gushiken, Cyntia Curcio, Luis Eduardo Coelho Andrade, Marcelo R.S. Briones |
| Rok vydání: |
2001 |
| Předmět: |
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| Zdroj: |
Proceedings of the National Academy of Sciences. 98:12103-12108 |
| ISSN: |
1091-6490
0027-8424 |
| DOI: |
10.1073/pnas.201182798 |
| Popis: |
Open reading frame expressed sequences tags (ORESTES) differ from conventional ESTs by providing sequence data from the central protein coding portion of transcripts. We generated a total of 696,745 ORESTES sequences from 24 human tissues and used a subset of the data that correspond to a set of 15,095 full-length mRNAs as a means of assessing the efficiency of the strategy and its potential contribution to the definition of the human transcriptome. We estimate that ORESTES sampled over 80% of all highly and moderately expressed, and between 40% and 50% of rarely expressed, human genes. In our most thoroughly sequenced tissue, the breast, the 130,000 ORESTES generated are derived from transcripts from an estimated 70% of all genes expressed in that tissue, with an equally efficient representation of both highly and poorly expressed genes. In this respect, we find that the capacity of the ORESTES strategy both for gene discovery and shotgun transcript sequence generation significantly exceeds that of conventional ESTs. The distribution of ORESTES is such that many human transcripts are now represented by a scaffold of partial sequences distributed along the length of each gene product. The experimental joining of the scaffold components, by reverse transcription–PCR, represents a direct route to transcript finishing that may represent a useful alternative to full-length cDNA cloning. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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