Heterogeneous and homogeneous electrochemiluminoimmunoassays of hTSH at disposable oxide-covered aluminum electrodes

Autor:	Sakari Kulmala, Jarkko Uolevi Eskola, Anna-Maria Spehar, Annika Nyman, Kari Loikas, Jouko Kankare, T. Ala-Kleme, Markus Håkansson
Rok vydání:	2002
Předmět:	Detection limit Chromatography medicine.diagnostic_test Aluminum electrode Chemistry Oxide Analytical chemistry Biochemistry Analytical Chemistry chemistry.chemical_compound Homogeneous Immunoassay medicine Environmental Chemistry Electrochemiluminescence Human thyroid Luminescence Spectroscopy
Zdroj:	Analytica Chimica Acta. 458:271-280
ISSN:	0003-2670
DOI:	10.1016/s0003-2670(02)00065-x
Popis:	Heterogeneous and homogeneous immunoassays of human thyroid stimulating hormone (hTSH) were developed on immunometric basis using aromatic Tb(III) chelates as electrochemiluminescent labels and varied types of disposable oxide-covered aluminum electrodes as the solid phase of the immunoassays. The long luminescence lifetime of the present labels allows the use of time-resolved electrochemiluminescence detection and provide the low detection limits of these labels and, thus, sensitive immunoassays. The primary antibody of immunometric immunoassays was coated upon aluminum oxide surface by physical absorption. In homogeneous immunoassays using 66l cell and 15 min incubation time, a linear calibration range of 0.25-324U/ml was obtained by applying only a single cathodic excitation pulse in the detection step of the assay. © 2002 Elsevier Science B.V. All rights reserved.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::371192a22617dce2d9d14a4bd5a759eb https://doi.org/10.1016/s0003-2670(02)00065-x Zobrazit plný text záznamu Full Text from ScienceDirect