Purification of Hemocyanin from Marine GastropodRapana Thomasianausing Ammonium Sulfate Precipitation Method

Autor:	K. Idakieva, I. Chakarska, P. Ivanova, A. Tchorbanov, I. Dobrovolov, L. Doumanova
Rok vydání:	2009
Předmět:	Ammonium sulfate Chromatography medicine.medical_treatment Hemocyanin Biology Gel permeation chromatography chemistry.chemical_compound Rapana thomasiana Column chromatography chemistry Biochemistry Hemolymph medicine Ultracentrifuge Ammonium sulfate precipitation Biotechnology
Zdroj:	Biotechnology & Biotechnological Equipment. 23:1364-1367
ISSN:	1314-3530 1310-2818
Popis:	The aim of this study was to develop an efficient and simple method for isolation of preparative amounts of pure hemocyanin (Hc) from the hemolymph of the marine gastropod Rapana thomasiana. The methods that are usually used, as ultracentrifugation and column chromatography, are expensive and impractical for the large-scale production of Hc. For the ammonium sulfate precipitation method, the concentrated hemolymph was twice precipitated by 38% saturation with crystalline ammonium sulfate. Rapana thomasiana hemocyanin (RtH) was isolated with good yield and high purity, as assessed by gel chromatography, SDSPAGE, transmission electron microscopy and absorption spectroscopy. This suggests that the ammonium sulfate precipitation is an efficient and useful purification method, suitable for a large-scale RtH preparation.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::370d6bc5cad6d9e168f6a05357bc7163 https://doi.org/10.1080/13102818.2009.10817671 Zobrazit plný text záznamu