Hyphenated Flash Chromatographic Separation and Isolation of Coumarins and Polymethoxyflavones from Byproduct of Citrus Juice Processing Industry
| Autor: | Bhimanagouda S. Patil, Ram M. Uckoo, Guddadarangavvanahally K. Jayaprakasha |
|---|---|
| Rok vydání: | 2013 |
| Předmět: |
chemistry.chemical_classification
Chloroform Chromatography Process Chemistry and Technology General Chemical Engineering Filtration and Separation General Chemistry Bergapten Flavones High-performance liquid chromatography Hexane chemistry.chemical_compound Tangeretin Column chromatography chemistry Acetone |
| Zdroj: | Separation Science and Technology. 48:1467-1472 |
| ISSN: | 1520-5754 0149-6395 |
| DOI: | 10.1080/01496395.2012.755696 |
| Popis: | The present study describes an efficient hyphenated flash chromatographic (FC) for the purification of coumarins and polymethoxy flavones (PMF's) from cold pressed grapefruit peel oil (GFO), a byproduct from citrus juice processing plant. GFO was subjected to silica column chromatography using sequential isocratic elution by hexane and chloroform to separate the waxes and phytochemicals enriched fractions, respectively. The chloroform extract was used for hyphenated FC separation using a gradient mobile phase of hexane and acetone. The peak separations were monitored at dual wavelengths of λ210 nm and λ340 nm. The individual fractions were analyzed by HPLC, pooled and concentrated resulting in isolation of two coumarins (auraptene and bergapten) and three PMF's (tangeretin, heptamethoxyflavone, and nobiletin). The purity and identity of the isolated compounds was confirmed by spectral analysis using high performance liquid chromatography, matrix-assisted laser desorption/Ionization-time of flight-mass spe... |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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