Functional analysis of a novel C-glycosyltransferase in the orchid Dendrobium catenatum
| Autor: | Zhong-Jian Liu, Yingyi Luo, Gang Wei, Xiaoyu Ji, Zhiyao Ren, Guo-Qiang Zhang, Zhenbin Jiao, Jing Zhang, Shengchang Tao, Zhouxi Lei, Yuchen Wang |
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| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
0301 basic medicine chemistry.chemical_classification Phloretin Flavonoid Isovitexin Vitexin Nothofagin Plant Science Horticulture Biology 01 natural sciences Biochemistry 03 medical and health sciences chemistry.chemical_compound 030104 developmental biology Flavonoid biosynthesis chemistry Apigenin Glycosyltransferase Genetics biology.protein 010606 plant biology & botany Biotechnology |
| Zdroj: | Horticulture Research. 7 |
| ISSN: | 2052-7276 2662-6810 |
| DOI: | 10.1038/s41438-020-0330-4 |
| Popis: | Flavonoids, which are a diverse class of phytonutrients, are used by organisms to respond to nearly all abiotic stresses and are beneficial for human health. Glycosyltransferase, used during the last step of flavonoid biosynthesis, is important in flavonoid enrichment. However, little is known about glycosyltransferase in the orchid Dendrobium catenatum (D. officinale). In this study, we isolated a novel C-glycosyltransferase (designated DcaCGT) from the orchid D. catenatum by identifying and analyzing 82 putative genes in the GT1 family. DcaCGT could specifically catalyze not only di-C-glycosylation but also O-glycosylation. Apart from the normal function of catalyzing 2-hydroxynaringenin and phloretin to the respective di-C-glycosides, DcaCGT also catalyzes apigenin to cosmosiin. Targeted metabolic profiling of the substrates (2-hydroxynaringenin, phloretin, and apigenin) and products (vitexin, isovitexin, vicenin-2, nothofagin, 3’,5’-di-C-glucosylphloretin, and cosmosiin) in different tissues showed that vicenin-2 was the most abundant product of this novel enzyme. Cosmosiin was detected in flowers and flower buds. We also established that DcaCGT functions expanded throughout the evolution of D. catenatum. Residual OGT activity may help D. catenatum resist drought stress. Our study illustrates the function, origin, and differentiation of DcaCGT and provides insights into glycosylation and molecular propagation processes, which can be used to improve the production of flavonoids by the cultivated medicinal plant D. catenatum. |
| Databáze: | OpenAIRE |
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