A photoactivatable Src homology 2 (SH2) domain
| Autor: | Tong Ju, Xi Song, Ronald L. Cerny, Xin Shang, Jiantao Guo, Wei Niu |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
biology Chemistry General Chemical Engineering General Chemistry Protein engineering SH2 domain Receptor tyrosine kinase SH3 domain Cell biology 03 medical and health sciences 030104 developmental biology Biochemistry biology.protein GRB2 Tyrosine Signal transduction Proto-oncogene tyrosine-protein kinase Src |
| Zdroj: | RSC Advances. 6:51120-51124 |
| ISSN: | 2046-2069 |
| Popis: | Src homology 2 (SH2) domains bind specifically to phosphotyrosine-containing motifs. As a regulatory module of intracellular signaling cascades, the SH2 domain plays important roles in the signal transduction of receptor tyrosine kinase pathways. In this work, we reported the construction of a photoactivatable SH2 domain through the combination of protein engineering and genetic incorporation of photo-caged unnatural amino acids. Significantly enhanced recognition of a phosphotyrosine-containing peptide substrate by the engineered SH2 domain mutant was observed after light-induced removal of the photo-caging group. Optical activation allows the control of protein and/or cellular function with temporal and spatial resolution. This photoactivatable SH2 domain could potentially be applied to the study of tyrosine phsophorylation-associated biological processes. |
| Databáze: | OpenAIRE |
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