Coat protein-mediated transgenic resistance in tomato against a IB subgroup Cucumber mosaic virus strain
| Autor: | S. K. Raj, D. Pratap, Sushil Kumar, Abhishek Sharma, Karmveer Kumar Gautam, Sunil Kumar Snehi |
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| Rok vydání: | 2012 |
| Předmět: | |
| Zdroj: | Phytoparasitica. 40:375-382 |
| ISSN: | 1876-7184 0334-2123 |
| Popis: | Transgenic tomato plants containing the coat protein (CP) gene of Cucumber mosaic virus (CMV) of subgroup IB were developed through Agrobacterium-mediated transformations. The progenies of transgenic plants showed the presence of transgene, its expression and translation of 26 KDa CP. The T1 and T2 generation plants were evaluated for resistance against challenge inoculations by a homologous strain of CMV. Visual observations of challenged transgenic plants categorized them into resistant, tolerant and susceptible as compared with untransformed control plants. Out of 33 plants of the T1 generation, 36.3% showed resistance and remained symptomless throughout their life, 48.4% showed tolerance which developed delayed symptoms of mild mosaic, and 15.1% showed susceptibility to CMV which developed severe systemic mosaic and leaf distortion symptoms after 30 days of virus challenge. Out of 120 plants of the T2 generation, 60% showed resistance, 26.6% were tolerant and only 13.3% were found susceptible to challenge inoculations of CMV. Resistant transgenic plants also showed less CP accumulation in systemic upper leaves as compared with challenged untransformed plants. In this study, CP of a CMV subgroup IB strain has demonstrated a significant level of resistance in transgenic tomato plants against the CMV strain. The strategy may be applied for better quality and productivity of tomato crops. |
| Databáze: | OpenAIRE |
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