Propionate Enhances Synthesis and Secretion of Bile Acids in Primary Cultured Rat HepatocytesviaSuccinyl CoA
| Autor: | Katsumi Imaizumi, Sedarnawati Yasni, Keizaburo Hirata, Michihiro Sugano |
|---|---|
| Rok vydání: | 1992 |
| Předmět: |
chemistry.chemical_classification
biology Bile acid Cholesterol medicine.drug_class Organic Chemistry General Medicine Butyrate Applied Microbiology and Biotechnology Biochemistry Analytical Chemistry chemistry.chemical_compound chemistry Valine biology.protein Propionate medicine Citrate synthase Succinyl-CoA Isoleucine Molecular Biology Biotechnology |
| Zdroj: | Bioscience, Biotechnology, and Biochemistry. 56:1894-1896 |
| ISSN: | 1347-6947 0916-8451 |
| DOI: | 10.1271/bbb.56.1894 |
| Popis: | To discover the role of propionate produced by colonic bacteria, this study examined the secretion of bile acids and cholesterol 7α-hydroxylase activity in the primary cultured hepatocytes. Addition of propionate (2 mM) to the medium for 48 h caused an increase in the bile acid secretion and enzyme activity, while acetate and butyrate had no significant influence. Bile acid secretion was increased by the addition of succinyl CoA and its precursor substances (α -ketoglutarate, valine, isoleucine, and methionine), but not malate and oxaloacetate, which are the metabolites of succinyl CoA. α -Ketoglutarate and valine also increased the activity of cholesterol 7α -hydroxylase. Since cholesterol 7α -hydroxylase is a microsomal cytochrome P-450 enzyme and the formation of δ-aminolevulinate from succinyl CoA in the mitochondria is the rate-controlling step for the subsequent synthesis of heme proteins, propionate may affect bile acid synthesis via elevation of mitochondrial succinyl CoA. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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