Identification of recombinant human EPO variants in greyhound plasma and urine by ELISA, LC-MS/MS and western blotting: a comparative study
| Autor: | John H. Vine, Mark Timms, Rohan Steel |
|---|---|
| Rok vydání: | 2015 |
| Předmět: |
0301 basic medicine
Darbepoetin alfa Chemistry 010401 analytical chemistry Pharmaceutical Science Cross reactions Epoetin alfa Urine 01 natural sciences Molecular biology 0104 chemical sciences Analytical Chemistry law.invention Blot 03 medical and health sciences 030104 developmental biology Erythropoietin law Lc ms ms medicine Recombinant DNA Environmental Chemistry Spectroscopy medicine.drug |
| Zdroj: | Drug Testing and Analysis. 8:164-176 |
| ISSN: | 1942-7603 |
| Popis: | The recombinant human erythropoietins epoetin alfa (Eprex®), darbepoetin (Aranesp®) and methoxy polyethylene glycol-epoetin beta (Mircera®) were administered to greyhounds for 7, 10 and 14 days respectively. Blood and urine samples were collected and analysed for erythropoietin by ELISA, LC-MS/MS and western blotting. Limits of confirmation in plasma for western blotting and LC-MS/MS methods ranged from a low of 2.5mIU/mL, and closely matched the sensitivity of ELISA screening. |
| Databáze: | OpenAIRE |
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