Effect of sperm DNA fragmentation on blastocyst formation rate and subsequent IVF outcome
| Autor: | Sankar Kumar Das, Krishna Kalita |
|---|---|
| Rok vydání: | 2022 |
| Předmět: | |
| Zdroj: | International Journal of Reproduction, Contraception, Obstetrics and Gynecology. 11:385 |
| ISSN: | 2320-1789 2320-1770 |
| DOI: | 10.18203/2320-1770.ijrcog20220054 |
| Popis: | Background: Male infertility associated with sperm DNA alteration has raised a new issue in assisted reproduction techniques (ARTs).Methods: It was a retrospective analytical study on 250 cases of routine IVF/ICSI performed at Swagat ART Centre from January 2017 to January 2020. We divided the patient according to the sperm DNA fragmentation index (DFI) as normal DFI≤15%, n=95, a moderate DFI≤30%, n=89, and a high DFI group >30%, n=66. Oocytes of each patient were almost equally divided and fertilization method was adopted as half IVF half ICSI or only ICSI in poor quality (oligo, astheno, teratozoospermia or with two or all three defect and compared the fertilization, cleavage, embryo formation, blastocyst formation, pregnancy and early embryo formation rate among these six groups. Results: Fertilization, cleavage, embryo formation, and clinical pregnancy rates were reported as higher in ≤15% DFI group of both IVF and ICSI-ET (87.3±26.2, 77.7±26.1, 68.2±28.8, 50.8 in IVF and 78.3±17.8, 70.3±31.2, 67.2±28.8, 57.6 respectively). Significant differences (p |
| Databáze: | OpenAIRE |
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