Characterization of Capsicum chlorosis virus infecting chilli (Capsicum annuum. L) in southern India
| Autor: | B. D. Haokip, S. K. Manoranjitham, Gandhi Karthikeyan, D. Alice, K. Nagendran, L. Rajendran |
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| Rok vydání: | 2021 |
| Předmět: |
0106 biological sciences
0301 basic medicine Veterinary medicine Spots Phylogenetic tree Sequence analysis Reassortment RNA Plant Science Horticulture Biology 01 natural sciences Virus 03 medical and health sciences 030104 developmental biology Agronomy and Crop Science Gene Recombination 010606 plant biology & botany |
| Zdroj: | European Journal of Plant Pathology. 160:637-647 |
| ISSN: | 1573-8469 0929-1873 |
| Popis: | During a field survey in Tamil Nadu (2014–16), chilli plants showing chlorotic and necrotic ring spots and necrotic streaks on leaves and stems were observed. Disease incidence ranged between 5 and 50% among five surveyed districts. RT-PCR assay using species specific primers detected 25% of samples infected with Capsicum chlorosis virus (CaCV) and 50% of samples infected with groundnut bud necrosis virus (GBNV). For simultaneous detection of CaCV and GBNV, duplex RT-PCR (dRT-PCR) has been validated. Complete nucleotide sequences of L-RNA, M-RNA, NSs and N genes of CaCV isolate (CaCV-TN-CBE) were determined.The L-RNA constitutes 8913 nt, M-RNA of 4848 nt, NSs gene of 1320 nt and the N gene of 828 nt. Sequence analysis showed that CaCV-TN-CBE isolate is closely related to China isolate based on L- and M- RNA whereas with Taiwan isolate based on S RNA. Also, based on nucleotide identity and phylogenetic analysis CaCV-TN-CBE isolate is different from the previously reported isolate from India. Further nucleotide sequences of L- and S- RNA have shown recombination events. This study suggested the isolate infecting chilli in Tamil Nadu might have evolved by undergoing recombination and reassortment evolutionary mechanisms. |
| Databáze: | OpenAIRE |
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