Negative association between autoantibodies against IL-17, IL-17/anti-IL-17 antibody immune complexes and destruction in rheumatoid arthritis
| Autor: | Alice Clement, Diane Razanajaona-Doll, Pierre Miossec, Jean-Jacques Pin, Ndiémé Ndongo-Thiam |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
biology business.industry medicine.medical_treatment Immunology Autoantibody Interleukin medicine.disease General Biochemistry Genetics and Molecular Biology Proinflammatory cytokine 03 medical and health sciences 030104 developmental biology Cytokine Rheumatology Antigen Rheumatoid arthritis medicine biology.protein Immunology and Allergy Interleukin 17 Antibody business |
| Zdroj: | Annals of the Rheumatic Diseases. 75:1420-1422 |
| ISSN: | 1468-2060 0003-4967 |
| Popis: | Autoantibodies against proinflammatory cytokines1 such as interleukin (IL)-1α are protective and a marker of good prognosis in rheumatoid arthritis (RA).2 ,3 They bind their antigen, that is, the cytokine, forming immune complexes (ICs). IL-17 is a new therapeutic target for a growing number of disorders,4 and levels of circulating bioactive IL-17 are associated with RA severity.5 Our objective was to define the contribution of anti-IL-17 autoantibodies and IL-17-anti-IL-17 ICs. A competitive ELISA was developed to measure anti-IL-17 autoantibodies. A positive control with added anti-IL-17 antibodies showed an inverse dose–response curve reflecting the competition, with no variation with irrelevant antibodies (figure 1A). Plasma of 30 healthy donors were first tested to determine the threshold (absorbance=0.9±0.1 at 1/2 dilution) with no variation between 1/4 and 1/8 dilutions, indicating an absence of anti-IL-17 autoantibodies. In contrast, they were detected in 36.6% of the 60 patients with RA (p |
| Databáze: | OpenAIRE |
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