Comparative in vitro study on the local tolerance and efficacy of benzalkonium chloride, myristalkonium chloride and nonoxynol-9 as active principles in vaginal contraceptives
| Autor: | François Verriere, Renata S. Tavares, Rita António Santos, Célia Gendron, Teresa Almeida-Santos, Ana Paula Sousa, Violaine Jabbour, Andreia F Silva, João Ramalho-Santos, Yana Mas, Maria Inês Alfaiate |
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| Rok vydání: | 2021 |
| Předmět: |
Active ingredient
030219 obstetrics & reproductive medicine biology business.industry Obstetrics and Gynecology Motility Pharmacology biology.organism_classification Sperm HeLa 03 medical and health sciences Benzalkonium chloride 0302 clinical medicine Reproductive Medicine medicine Pharmacology (medical) Nonoxynol-9 030212 general & internal medicine business Acrosome medicine.drug Hormone |
| Zdroj: | The European Journal of Contraception & Reproductive Health Care. 26:334-342 |
| ISSN: | 1473-0782 1362-5187 |
| Popis: | BACKGROUND Spermicides have been identified as a potentially attractive alternative to hormonal contraceptives and/or intrauterine devices. Thus, this study aimed evaluating the efficacy and local tolerance of benzalkonium chloride (BKC) and myristalkonium chloride (MKC) contained in Pharmatex® vaginal formulations and compare them with nonoxynol-9 (N-9), the most common active ingredient in topical vaginal contraceptives. METHODS Human normozoospermic samples were assessed for motility, viability, acrosome status and penetration ability after exposure to control, N-9 or different BKC and MKC doses for 0 and 10 minutes. Local tolerance on HeLa cells was evaluated by the Trypan-blue and MTT assays. RESULTS Exposure to BKC and MKC reduced acrosome integrity while promoting total immobilisation and complete loss of sperm viability (p |
| Databáze: | OpenAIRE |
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