Tumor necrosis factor alpha (rhTNF) fails to stimulate angiogenesis in the rabbit cornea

Autor:	David R. Knighton, A. Marika Stone, Bryan D. Jones, Julie C. Schultz, Gregg D. Phillips, Mark J. Lisowski, Margot L. Goodkin
Rok vydání:	1996
Předmět:	Hydron Angiogenesis Biological activity Biology Pharmacology Agricultural and Biological Sciences (miscellaneous) In vitro Neovascularization chemistry.chemical_compound chemistry In vivo Immunology medicine Tumor necrosis factor alpha Anatomy medicine.symptom Cytotoxicity
Zdroj:	The Anatomical Record. 245:53-56
ISSN:	1097-0185 0003-276X
DOI:	10.1002/(sici)1097-0185(199605)245:1<53::aid-ar9>3.0.co;2-g
Popis:	BACKGROUND The objective of this study was to thoroughly examine the in vivo angiogenesis activity of human recombinant tumor necrosis factor alpha (rhTNF). METHODS rhTNF (0.5 ng to 1.0 microgram) was incorporated into the slow release polymers Hydron or HYPAN and implanted into the rabbit cornea. Release of biologically active rhTNF from the polymers was determined with the L929 cytotoxicity assay. RESULTS All concentrations tested failed to elicit capillary formation beyond that observed for controls. Less than 2% of the rhTNF was released from the Hydron over 7 days. HYPAN released five times the amount of rhTNF in vitro, but even at doses of 500 ng (104.3 ng suggested release) no angiogenesis was stimulated. CONCLUSIONS Under the circumstances tested, rhTNF is not angiogenic in vivo.
Databáze:	OpenAIRE
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