Isolation and characterization of microsatellite DNA loci from the golden cuttlefish, Sepia esculenta Hoyle (Cephalopoda)

Autor:	Xiaodong Zheng, Nobuhiko Taniguchi, Minoru Ikeda, Anna Alexandrovna Barinova
Rok vydání:	2006
Předmět:	Cuttlefish Genetics education.field_of_study Ecology Population Locus (genetics) Biology Biochemistry General Biochemistry Genetics and Molecular Biology law.invention law Evolutionary biology Genetic variation Microsatellite Allele education Sepia esculenta Polymerase chain reaction
Zdroj:	Molecular Ecology Notes. 7:40-42
ISSN:	1471-8286 1471-8278
DOI:	10.1111/j.1471-8286.2006.01518.x
Popis:	The first microsatellite markers were isolated from the golden cuttlefish, Sepia esculenta Hoyle. Eleven primer sets were designed to amplify the marker sequences via polymerase chain reaction. The 45–50 individuals from one wild population in the coastal waters of Ehime Prefecture, Japan were used to screen polymorphism in the 11 microsatellite loci. All the microsatellite loci were polymorphic, with the range of alleles from seven to 27 per locus. The observed and expected heterozygosities ranged from 0.380 to 0.980 and from 0.654 to 0.940, respectively. These marker loci except for one locus showing significant deviation from Hardy–Weinberg equilibrium will be useful for the assessment of genetic variation and population structure of this species.
Databáze:	OpenAIRE
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