Mass-sensing, multianalyte microarray immunoassay with imaging detection

Autor:	John W. Silzel, Bibijana Cercek, Tsong Tsay, Charles L. Dodson, Robert J. Obremski
Rok vydání:	1998
Předmět:	Fluorescence-lifetime imaging microscopy Analyte Chromatography medicine.diagnostic_test biology Chemistry Ligand binding assay Biochemistry (medical) Clinical Biochemistry Analytical chemistry Fluorescence Fluorescence spectroscopy Immunoassay Reagent medicine biology.protein Avidin
Zdroj:	Clinical Chemistry. 44:2036-2043
ISSN:	1530-8561 0009-9147
DOI:	10.1093/clinchem/44.9.2036
Popis:	Miniaturization of ligand binding assays may reduce costs by decreasing reagent consumption, but it is less apparent that miniaturized assays can simultaneously exceed the sensitivity of macroscopic techniques by analyte “harvesting” to exploit the total analyte mass available in a sample. Capture reagents (avidin or antibodies) immobilized in 200-μm diameter zones are shown to substantially deplete analyte from a liquid sample during a 1–3-h incubation, and the assays that result sense the total analyte mass in a sample rather than its concentration. Detection of as few as 105 molecules of analyte per zone is possible by fluorescence imaging in situ on the solid phase using a near-infrared dye label. Single and multianalyte mass-sensing sandwich array assays of the IgG subclasses show the sensitivity and specificity of ELISA methods but use less than 1/100 the capture antibody required by the 96-well plate format.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::36bdd4c2ca8805579167dadb32542e76 https://doi.org/10.1093/clinchem/44.9.2036 Zobrazit plný text záznamu