Specific Environmental Temperature and Relative Humidity Conditions and Grafting Affect the Persistence and Dissemination of Salmonella enterica subsp. enterica Serotype Typhimurium in Tomato Plant Tissues
| Autor: | Gireesh Rajashekara, Sally A. Miller, Yosra A. Helmy, Loic Deblais, Cláudio Maurício Vrisman, Anna L. Testen, Dipak Kathayat, Alejandra M. Jimenez Madrid |
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| Rok vydání: | 2019 |
| Předmět: |
Abiotic component
0303 health sciences Salmonella Ecology biology 030306 microbiology Inoculation fungi food and beverages biology.organism_classification medicine.disease_cause Applied Microbiology and Biotechnology Persistence (computer science) 03 medical and health sciences Horticulture Salmonella enterica medicine Preharvest Salmonella enterica subsp. enterica Phyllosphere 030304 developmental biology Food Science Biotechnology |
| Zdroj: | Applied and Environmental Microbiology. 85 |
| ISSN: | 1098-5336 0099-2240 |
| DOI: | 10.1128/aem.00403-19 |
| Popis: | Little is known about the abiotic factors contributing to the preharvest persistence of Salmonella in tomato tissues. Therefore, we investigated the effects of specific environmental conditions and contamination methods on the persistence and dissemination of Salmonella enterica subsp. enterica serotype Typhimurium (JSG626) in tomato plants. When plants were sprayed on the leaves with a JSG626-contaminated solution, JSG626 persistence in the phyllosphere (bacteria located on the surface of the inoculated foliage and stem tissues) was lower at higher temperatures (30°C day/25°C night) than at lower temperatures (20°C day/15°C night). However, wounding cotyledons with contaminated tools improved JSG626 persistence and the internalization rate (2.27%) in planta compared to spray inoculation (0.004%). The systemic dissemination of JSG626 to other tissues increased when contaminated plants were grown under low relative humidity ( IMPORTANCE Tomatoes are one of the most widely produced vegetables around the world; however, fresh tomatoes have been connected to multiple wide-scale salmonellosis outbreaks over the past decades. Salmonella is commonly found in the environment and can persist in hostile conditions for several weeks before being internalized into plant tissues, where it is protected from conventional sanitation methods. In addition to biotic factors (host, inoculum size, and phytobiome), abiotic factors (environmental conditions) may affect the persistence of Salmonella in crop production. This study demonstrates that specific environmental conditions, the inoculation method, and the inoculum density affect the persistence and dissemination of JSG626 in tomato plant tissues. Our findings enhance the understanding of interactions between Salmonella enterica and fresh produce and may lead to the development of novel management practices on farms. |
| Databáze: | OpenAIRE |
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