Molecular genetic discrimination of Buffel grass genotypes and F1 hybrids for breeding purposes using amplified fragment length polymorphism analyses

Autor:	Nacira Muñoz, A. Ribotta, C. Luna, S. Griffa, K. Grunberg, S. Lanza Castelli, E. Biderbost, E. López Colomba, D. Díaz
Rok vydání:	2006
Předmět:	Genetics Veterinary medicine Jaccard index food and beverages Management Monitoring Policy and Law Biology biology.organism_classification Cenchrus ciliaris Genetic similarity Apomixis Genotype Amplified fragment length polymorphism Cultivar Agronomy and Crop Science Hybrid
Zdroj:	Grass and Forage Science. 61:454-458
ISSN:	1365-2494 0142-5242
DOI:	10.1111/j.1365-2494.2006.00546.x
Popis:	Buffel grass (Cenchrus ciliaris L.) is an apomictic species that is widely adapted to arid and semi-arid, tropical and warm climates. Six apomictic cultivars used as male parents, one sexual line used as the female parents and 15 putative F1 genotypes were studied using the amplified fragment length polymorphism (AFLP) technique for their evaluation and recognition. The NTSYS programme and Jaccard's Index were used to establish the genetic similarity; and a phenogram was constructed. A total of 152 bands was recorded. Three primer combinations were sufficient to identify the genotypes. The maximum proportion of polymorphisms was 0·835, and 0·510 of the polymorphisms consisted in individual bands that strictly diagnosed unique genotypes. Texas 4464 and Americana cultivars were the most different from the sexual line, indicating that they might be the best genotypes for future crossing. Two hybrids were identified among F1 material to demonstrate the usefulness of the AFLP technique in characterizing genotypes and recognizing hybrids in Buffel grass for breeding purposes.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::36b2ee6f24349f363ad74c0a2f15b4e8 https://doi.org/10.1111/j.1365-2494.2006.00546.x Zobrazit plný text záznamu Plný text ve formátu PDF