Isolation, Identification, Antibacterial Effects of Antibiotic Drugs, and Chinese Herbal Extracts to the Pathogenic Bacteria of Swollen Abdomen from Scophthalmus maximus in Vitro
| Autor: | Xuan Wu, Guoxia Zhu, Peng Zhou, Yanbin Ji, Dongqing Bai, Zhichao Jia |
|---|---|
| Rok vydání: | 2013 |
| Předmět: |
biology
Cefepime Pathogenic bacteria Aztreonam Sulbactam biochemical phenomena metabolism and nutrition bacterial infections and mycoses biology.organism_classification medicine.disease_cause Microbiology Ciprofloxacin Stenotrophomonas maltophilia chemistry.chemical_compound chemistry polycyclic compounds medicine bacteria Cefuroxime medicine.drug Piperacillin |
| Zdroj: | Proceedings of the 2012 International Conference on Applied Biotechnology (ICAB 2012) ISBN: 9783642379215 |
| Popis: | The pathogenic bacteria was isolated from Turbot (Scophthalmus maximus) with swollen abdomen, and proved to be the pathogenic bacteria by the infection experiment. In this research, VITEK-2 compact system was used to identify the pathogenic bacteria, and test the antibacterial effects of antibiotic drugs, paper diffusion method was used to evaluate the antibacterial effects of three kinds of Chinese herbal extracts on the pathogenic bacteria of swollen abdomen from Turbot in Vitro.The results showed that the pathogenic bacteria of swollen abdomen from Turbot was stenotrophomonas maltophilia, and the separation purity was 99 %. Without Trimethoprim, S. maltophilia had no CLSI salient point to the antibiotics of Ampicillin, Amikacin, Aztreonam, Cefazolin, Cefepime, Cefuroxime, Cefuroxime Axetil, Cefotetan, Ceftazidime, Ceftriaxone, Ciprofloxacin, Piperacillin, Imipenem, Gentamicin, Levofloxacin, Nitrofurantoin, Sulbactam, Tazobactam. Extract of the scutellaria and honeysuckle had different antibacterial effects to the S. maltophilia, and then the best bacteriostatic (MIC) and bactericidal (MBC) effects were 3.125 mg/mL and 6.25 mg/mL, respectively. However, the best MIC and MBC effects to extract from polygonum cuspidate were 100 mg/mL and 200 mg/mL, MIC and MBC effects lower than that extract from the scutellaria and honeysuckle. The results can provide reference materials for the clinical use of aquaculture and aquatic animal health cultivation. |
| Databáze: | OpenAIRE |
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