Amino acid sequence of the L-arabinose-binding protein from Escherichia coli B/r
| Autor: | M A Hermodson, R W Hogg |
|---|---|
| Rok vydání: | 1977 |
| Předmět: |
chemistry.chemical_classification
Protease Chymotrypsin medicine.medical_treatment Binding protein Cell Biology Biology medicine.disease_cause Biochemistry Carboxypeptidase Amino acid chemistry.chemical_compound chemistry medicine biology.protein Cyanogen bromide Molecular Biology Escherichia coli Peptide sequence |
| Zdroj: | Journal of Biological Chemistry. 252:5135-5141 |
| ISSN: | 0021-9258 |
| DOI: | 10.1016/s0021-9258(17)40167-0 |
| Popis: | The amino acid sequence of the L-arabinose-binding protein of Escherichia coli B/r was determined by sequenator analyses of reduced and S-pyridylethylated L-arabinose-binding protein and fragments derived by chemical and enzymatic cleavage of the native protein. The fragments were the products of cleavage by cyanogen bromide. BNPS-skatole, hydroxylamine, mild acid hydrolysis, limited trypsin digestion, chymotrypsin subdigestion, and subdigestion with Staphylococcus aureus protease V8. The COOH-terminal sequence was determined using bovine carboxypeptidases A and B and amino acid analyses. The L-arabinose-binding protein was determined to contain 306 amino acid residues, the sequence of which is presented below. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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