Autor: Anthony R. Grivell, Marlene B. Grivell, John W. Phillips, Michael N. Berry
Rok vydání: 1997
Předmět:
Zdroj: Cell Biology and Toxicology. 13:223-233
ISSN: 0742-2091
DOI: 10.1023/a:1007402505482
Popis: The first technique for large-scale preparation of isolated hepatocytes was described in 1953 and involved perfusion of rat liver under pressure with a Ca2+-free solution containing a chelating agent. Various modifications of this technique were in use over the next ten years, until it was demonstrated that cells prepared in this manner were grossly damaged, losing most of their cytoplasmic enzymes during the preparative procedure. The successful preparation of intact isolated hepatocytes by collagenase-treatment of liver was achieved in 1967, and the widespread use of intact hepatocyte suspensions was accelerated by the development soon after of high-yield preparative techniques involving perfusion of the liver with a medium containing collagenase.
Databáze: OpenAIRE
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