| Autor: |
Cong Wang, Zhi Li, Xiao Zhu, Yue Ding, Wenjia Duan, Wanbing Sun, Yiqing Jiang, Ming Chen, Jiayi Hu, Zheping Cai, Jing Zhao, Zhen Fan, Faming Zheng, Xingyu Zhou, Fang Xie, Jianping Zhang, Yihui Guan, Xiao Xiao, Liang Chen, Cong Li, Ying Mao |
| Rok vydání: |
2023 |
| DOI: |
10.21203/rs.3.rs-1868381/v1 |
| Popis: |
Resective surgery is used for long-term seizure control in drug-resistant focal epilepsy when the epileptic focus (EF) can be accurately delineated and removed. However, as the “gold standard” technique for mapping EF intraoperatively, electroencephalography is laborious, time-consuming and highly vulnerable to anaesthesia. Here, we demonstrated hypochlorous acid (HOCl), a bioproduct of activated proinflammatory microglia, can be reliable biomarkers for EF localization. With a newly developed HOCI ratiometric Raman nanosensor, ultraHOCls, we can accurately locate EF in live kainic-acid-induced mice by visualizing proinflammatory microglia ignoring the interference of anaesthesia. Moreover, ultraHOCls provided a 1-hour stable imaging time-window for locating EF, which is more than 100 times longer than single neuroelectric activity recorded by electroencephalography. UltraHOCls-guided surgery showed a 72% reduction in total seizure time in mice in comparison to electroencephalography-guided surgery. This work provides an alternative strategy for locating EF intraoperatively and holds promise in improving surgical outcomes in epilepsy patients. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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