Protein kinase C stimulation enhances the process formation of adult oligodendrocytes and induces proliferation
| Autor: | J. Schröter, H. H. Althaus, P. E. Spoerri, Sabine Klöppner, A. Rohmann, Peter Schwartz, Volker Neuhoff |
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| Rok vydání: | 1991 |
| Předmět: |
0303 health sciences
Stimulation Biology Oligodendrocyte In vitro Amiloride Cell biology 03 medical and health sciences Cellular and Molecular Neuroscience 0302 clinical medicine medicine.anatomical_structure Biochemistry Calmodulin Pathway medicine Neuroglia Protein kinase A 030217 neurology & neurosurgery Protein kinase C 030304 developmental biology medicine.drug |
| Zdroj: | Journal of Neuroscience Research. 29:481-489 |
| ISSN: | 0360-4012 |
| DOI: | 10.1002/jnr.490290408 |
| Popis: | Oligodendrocytes (OL) isolated from adult pig brains regenerate their processes and form a network of fibers after 14-18 days in vitro (DIV). Stimulation of protein kinase C (Pk-C) by tumour promoters such as 12-O-tetradecanoylphorbol-13-acetate (TPA) produces at day 7 in vitro a similar network after only 20 hr. H-7, an inhibitor of Pk-C, as well as amiloride, which inhibits the subsequent Na+/H+ exchange, reversibly suppress this effect. Activation of the protein kinase A or calmodulin pathway do not result in an increased OL process production. Furthermore, TPA induced proliferation in a subpopulation of OL. We conclude that the stimulation of Pk-C is of utmost importance for OL regeneration. |
| Databáze: | OpenAIRE |
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