Expression, purification and biophysical characterization of recombinant Streptomyces violaceoruber phospholipase PLA2 overproduced in Pichia pastoris

Autor:	S. Y. Filkin, E. G. Sadykhov, A. A. Zenin, A N Fedorov, Vladimir Popov, A. V. Lipkin, A A Sichev, D S Bityak, N. V. Chertova
Rok vydání:	2020
Předmět:	biology Chemistry General Medicine Streptomyces violaceoruber Phospholipase biology.organism_classification Biochemistry Secretory Phospholipase A2 Pichia pastoris law.invention Phospholipase A2 law biology.protein Recombinant DNA lipids (amino acids peptides and proteins) Biotechnology
Zdroj:	Preparative Biochemistry & Biotechnology. 50:549-555
ISSN:	1532-2297 1082-6068
DOI:	10.1080/10826068.2020.1712657
Popis:	Aim: The main purpose of this work was to develop new protocols for high yield purification of secretory phospholipase A2 (PLA2) and to investigate its biophysical properties.Materials and methods:...
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::3664f367cba8fd263844f3d78b036760 https://doi.org/10.1080/10826068.2020.1712657 Zobrazit plný text záznamu