Identification of microRNAs from Zn-treated Solanum nigrum roots by small RNA sequencing
| Autor: | Ping Zhang, Jianping Gao, Jingjing Zhao, Jin Xu, Zhixia Xie, Ruling Wang |
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| Rok vydání: | 2016 |
| Předmět: |
0106 biological sciences
0301 basic medicine Programmed cell death Small RNA biology Phenylpropanoid Physiology Catabolism Plant physiology Plant Science Solanum nigrum biology.organism_classification medicine.disease_cause 01 natural sciences 03 medical and health sciences 030104 developmental biology Biochemistry Botany Zinc toxicity microRNA medicine Agronomy and Crop Science 010606 plant biology & botany |
| Zdroj: | Acta Physiologiae Plantarum. 39 |
| ISSN: | 1861-1664 0137-5881 |
| DOI: | 10.1007/s11738-016-2337-x |
| Popis: | MicroRNAs (miRNAs) play key roles in regulating zinc (Zn) toxicity tolerance in plants. Solanum nigrum is a typical Zn/Cd-accumulating plant that has a high Zn/Cd tolerance. Despite their importance, no miRNAs have been identified from S. nigrum thus far. In this study, small RNA sequencing was used to identify Zn-responsive miRNAs in S. nigrum roots. We identified 176 differentially expressed miRNAs in S. nigrum roots in response to Zn toxicity. We also found that all these differentially expressed Zn-miRNAs simultaneously respond to the exogenous NO donor sodium nitroprusside (SNP), indicating that NO is involved in Zn-mediated miRNA expression in S. nigrum. These differentially expressed miRNAs are involved in regulating the following processes in S. nigrum roots: phenylpropanoid catabolic and metabolic processes; lignin catabolic and metabolic processes; and programmed cell death in response to ROS. These results suggest that these miRNAs play key roles in the Zn toxicity tolerance of S. nigrum. |
| Databáze: | OpenAIRE |
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