Sensitive and selective determination of metabolically formed trans -dihydrodiols and phenols of benzo[ a ]pyrene in water and urine samples by HPLC with amperometric detection

Autor: E. Fischer, G. Henze, K. L. Platt
Rok vydání: 1998
Předmět:
Zdroj: Fresenius' Journal of Analytical Chemistry. 360:95-99
ISSN: 1432-1130
0937-0633
DOI: 10.1007/s002160050649
Popis: A method has been developed to separate hydroxylated metabolites of the carcinogenic polycyclic aromatic hydrocarbon benzo[a]pyrene, i. e. trans-4,5-, 7,8-, 9,10-dihydrodiol and 1-, 3-, 7-, and 9-phenol, by HPLC with amperometric detection employing an isocratic methanol/water eluent (70:30, v/v) containing 0.5 g/L sulfuric acid and 1 g/L lithium perchlorate. Compared with the usually applied fluorescence (λex = 265 nm, λem = 460 nm) and ultraviolet (λ = 265 nm) detection, the amperometric technique is about 2–12 times more sensitive for the determination of all metabolites investigated. The method was applied to the determination of the seven metabolites of benzo[a]pyrene in different water samples and in urine after solid-phase extraction (SPE). The results obtained by HPLC with amperometric detection after SPE enrichment from an aqueous extract of a soil sample and from the urine of a rat intragastrically treated with benzo[a]pyrene agreed well with the values determined with fluorescence and/or UV detection.
Databáze: OpenAIRE
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