Liquid-chromatographic determination of urinary riboflavin

Autor:	H K Naito, V J Gatautis
Rok vydání:	1981
Předmět:	Chromatography Chemistry Elution Urinary system Biochemistry (medical) Clinical Biochemistry Linear relation food and beverages Urine specimen Riboflavin Fluorescence
Zdroj:	Clinical Chemistry. 27:1672-1675
ISSN:	1530-8561 0009-9147
DOI:	10.1093/clinchem/27.10.1672
Popis:	We describe a method for determining urinary riboflavin by "high-pressure" liquid chromatography, with fluorometric detection. An aliquot of a 24-h urine specimen is injected directly into the chromatograph and the natural fluorescence of riboflavin is measured as the compound is eluted. Interference from other fluorophores is obviated because of differing retention times. Urinary components other than riboflavin and its analogs or degradation products exhibit no significant fluorescence at the wavelengths used (450 nm excitation, 530 nm emission). Analytical recovery of added riboflavin was 96.5 (SD 1.1)%. The CV within-run was 0.7%, between-run it was 4.3%. Concentrations of riboflavin as low as 10 micrograms/L are readily detected, with a linear relation of response to concentration to at least 2000 micrograms/L.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::365353f35ba5ca08d4c7ad4e72cf3b81 https://doi.org/10.1093/clinchem/27.10.1672 Zobrazit plný text záznamu