Autor: Mikhaĭlova Vk, Repin Vladimir E, L. I. Puchkova, Ushakova Ta, Krivopalova Gn, Serov Gd
Rok vydání: 2002
Předmět:
Zdroj: Applied Biochemistry and Microbiology. 38:15-19
ISSN: 0003-6838
DOI: 10.1023/a:1013236218350
Popis: A new method of testing restriction nucleases is proposed. This method is based on high-temperature treatment of crude cell extracts. Disrupted cells were heated at 50–60°C, centrifuged, and assayed for restrictases. This method provides the opportunity for screening new enzymes in microbial strains enriched with nonspecific restrictases. High-temperature treatment of cell extracts of certain producers reduces the number of steps of the procedure used for isolating high-purity restrictases; the resulting preparations are capable of maintaining high enzymatic activity during long-term storage. It was shown that high-temperature treatment can be applied not only to thermophilic but also to mesophilic strains of microorganisms of different taxa.
Databáze: OpenAIRE
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