Extraction of Fat from Ground Beef for Nutrient Analysis Using Analytical Supercritical Fluid Extraction

Autor:	Jerry W. King, Floyd K. McKeith, Fred J. Eller, Charles R. Stites, James H. Johnson, Janet M. Snyder
Rok vydání:	1996
Předmět:	Hexane Solvent chemistry.chemical_compound Hydrolysis Chromatography Chemistry Extraction (chemistry) Supercritical fluid extraction General Chemistry Solid phase extraction Gas chromatography General Agricultural and Biological Sciences Hydrolysate
Zdroj:	Journal of Agricultural and Food Chemistry. 44:2700-2704
ISSN:	1520-5118 0021-8561
DOI:	10.1021/jf960069j
Popis:	A supercritical fluid extraction (SFE) method using CO2 has been developed for the removal analysis of fat from ground beef samples for nutritional analysis. The SFE procedure was coupled with a solid phase extraction (SPE) disk for the isolation of fats from acid hydrolyzed ground beef samples prior to SFE. The meat hydrolysate is filtered and collected on the reversed-phase SPE disk which is then subjected to SFE. The extracted fat is then transesterified to fatty acid methyl esters (FAMEs), and total, saturated, and cis-monounsaturated fats were determined by gas chromatography. The SFE method was tested on two commercial extractors and the results compared to a solvent-based (ether/hexane) extraction method. No significant differences were found between the results obtained by the solvent-based and the SC-CO2 methods. The SFE method proved a suitable replacement for a traditional organic solvent extraction methods, thereby eliminating the use and costs associated with solvent disposal as well as the e...
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::363477a9a48f072c74f458a0e4708dc5 https://doi.org/10.1021/jf960069j Zobrazit plný text záznamu