Loss of Etv5 Decreases Proliferation and RET Levels in Neonatal Mouse Testicular Germ Cells and Causes an Abnormal First Wave of Spermatogenesis1
| Autor: | Kenneth M. Murphy, Gail C. Ekman, Chris E. Hostetler, Natalia V. Kostereva, Daryl D. Meling, Marie Claude Hofmann, Kay Carnes, Gaurav Tyagi, Michael D. Griswold, Paul S. Cooke, Carla M.K. Morrow, Rex A. Hess |
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| Rok vydání: | 2009 |
| Předmět: |
endocrine system
medicine.medical_specialty Receptor complex Cell Biology General Medicine Biology Testicle Sertoli cell Andrology medicine.anatomical_structure Endocrinology Reproductive Medicine Internal medicine medicine Glial cell line-derived neurotrophic factor biology.protein Stem cell Spermatogenesis Germ cell Laser capture microdissection |
| Zdroj: | Biology of Reproduction. 81:258-266 |
| ISSN: | 1529-7268 0006-3363 |
| DOI: | 10.1095/biolreprod.108.075200 |
| Popis: | Mice that are ets variant gene 5 (ETV5) null (Etv5 � /� ) undergo the first wave of spermatogenesis but lose all spermatogonial stem cells (SSCs) during this time. The SSC loss in Etv5 � /� mice begins during the neonatal period, suggesting a role for ETV5 in SSC self-renewal during this period. Herein, we show that Etv5 mRNA was present in perinatal mouse testis and that ETV5 was expressed in fetal Sertoli cells and by germ cells and Sertoli cells during the neonatal period. Transplantation of Etv5 � /� germ cells failed to establish spermatogenesis in W/W v mice testes, indicating that germ cell ETV5 has a key role in establishment or self-renewal of transplanted SSCs. The SSC self-renewal is stimulated by glial cell-derived neurotrophic factor (GDNF) acting through the RET/GDNF family receptor alpha 1 (GFRA1) receptor complex in SSCs. Immunohistochemistry, quantitative PCR, and laser capture microdissection revealed decreased RET mRNA and protein expression in spermatogonia of neonatal |
| Databáze: | OpenAIRE |
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