Differential phagocytosis ofLeishmania mexicanapromastigotes and amastigotes by monocyte-derived dendritic cells
| Autor: | Arturo A. Wilkins-Rodríguez, Jesús Argueta-Donohué, Laila Gutiérrez-Kobeh, M. Magdalena Aguirre-García |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
medicine.diagnostic_test biology Phagocytosis 030106 microbiology Immunology Leishmaniasis medicine.disease biology.organism_classification Microbiology Leishmania mexicana Flow cytometry Antibody opsonization 03 medical and health sciences 030104 developmental biology Immune system Virology parasitic diseases medicine Amastigote Opsonin |
| Zdroj: | Microbiology and Immunology. 60:369-381 |
| ISSN: | 0385-5600 |
| DOI: | 10.1111/1348-0421.12325 |
| Popis: | Leishmania species are dimorphic protozoan parasites that live and replicate in the gut of sand flies as promastigotes or in mammalian hosts as amastigotes. Different immune cells, including DCs, and receptors differ in their involvement in phagocytosis of promastigotes and amastigotes and in recognition of different Leishmania species. In the case of L. mexicana, differences in phagocytosis of promastigotes and amastigotes by DCs and participation of C-type lectin receptors (CLRs) have not been established. In the present study, flow cytometry and confocal microscopy were used to investigate the phagocytosis by monocyte-derived dendritic cells (moDCs) of L. mexicana promastigotes and amastigotes in the presence or absence of immune serum during various periods of time. Blocking antibodies against mannose receptors and DC-SIGN were used to explore the participation of these receptors in the phagocytosis of L. mexicana by moDC. The major differences in interactions of L. mexicana promastigotes and amastigotes with moDC were found to occur within the first 3 hr, during which phagocytosis of promastigotes predominated as compared with opsonization of promastigotes and amastigotes. However, after 6 hr of incubation, opsonized promastigotes were preferentially phagocytosed as compared with unopsonized promastigotes and amastigotes and after 24 hr of incubation there were no differences in the phagocytosis of promastigotes and amastigotes. Finally, after 3 hr incubation, DC-SIGN was involved in the phagocytosis of promastigotes, but not of amastigotes. |
| Databáze: | OpenAIRE |
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