Composite MF@Ag-NPs microspheres for label-free quantitative detection of uric acid
| Autor: | Xu Dong, Ruimin Li, Christian G. Schäfer, Fang Wang |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
Reproducibility
Microscope Materials science Composite number technology industry and agriculture Substrate (chemistry) Nanotechnology 02 engineering and technology Surface-enhanced Raman spectroscopy 010402 general chemistry 021001 nanoscience & nanotechnology 01 natural sciences 0104 chemical sciences law.invention Colloid and Surface Chemistry law Molecule Particle 0210 nano-technology Biosensor |
| Zdroj: | Colloids and Surfaces A: Physicochemical and Engineering Aspects. 523:1-8 |
| ISSN: | 0927-7757 |
| DOI: | 10.1016/j.colsurfa.2017.03.042 |
| Popis: | A convenient and easy-scalable synthetic strategy to achieve ultrasensitive surface enhanced Raman spectroscopy (SERS) substrates for single particle detection of targeting molecules is reported. Melamine-formaldehyde (MF) microspheres with variable sizes were synthesized and decorated with Ag nanoparticles (NPs) through an in-situ chemical deposition to obtain composite MF@Ag-NPs microspheres. Individual MF@Ag-NPs microspheres were screened under a microscope and used to examine their single particle SERS properties by using four different probe molecules. The experimental results demonstrated that as-prepared SERS substrates displayed a dramatically enhanced SERS activity and sensitivity as well as an extraordinary stability and reproducibility of the SERS signal. Moreover, we also showed a first example of single MF@Ag-NPs microsphere for quantitative trace detection of uric acid (UA) as biomarker based on the label-free strategy, making them a promising candidate as an ideal substrate for applications in fields of biosensor, trace analysis and biomedical diagnostics. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect