Glutamine is not an essential amino acid for Sf-9 insect cells
| Autor: | Lena Häggström, Jan Ljunggren, Anna-Karin Ramqvist, Lars Öhman, Maria Alarcon |
|---|---|
| Rok vydání: | 1996 |
| Předmět: |
chemistry.chemical_classification
viruses fungi Bioengineering Sf9 General Medicine Biology Spodoptera biology.organism_classification Applied Microbiology and Biotechnology law.invention Glutamine chemistry.chemical_compound chemistry Biochemistry law Cell culture Recombinant DNA Trichoplusia Ammonium Essential amino acid Biotechnology |
| Zdroj: | Biotechnology Letters. 18:765-770 |
| ISSN: | 1573-6776 0141-5492 |
| DOI: | 10.1007/bf00127885 |
| Popis: | Spodoptera frugiperda (Sf-9) insect cells are fully capable of growth and proliferation in a glutamine, glutamate and aspartate-free medium, provided that ammonium ions are supplied. S. frugiperda (Sf-21) and Mamestra brassicae cells (IZD-MB-0503) also grow in glutamine-free media but not Trichoplusia ni cells (BTI-TN 5B1-4). The yield of β-galactosidase in Sf-9 cells infected with a recombinant baculovirus under glutamine-free conditions was even higher than the yield obtained in glutamine containing cultures. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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