Determination of 21 drugs in oral fluid using fully automated supported liquid extraction and UHPLC-MS/MS
| Autor: | Åse Marit Leere Øiestad, Thomas Berg, Ragnhild Skari, Anja Valen, Dag Helge Strand |
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| Rok vydání: | 2016 |
| Předmět: |
Detection limit
Chromatography Chemistry 010401 analytical chemistry Extraction (chemistry) Pharmaceutical Science Ion suppression in liquid chromatography–mass spectrometry Urine Tandem mass spectrometry Mass spectrometry 01 natural sciences 0104 chemical sciences Analytical Chemistry 03 medical and health sciences 0302 clinical medicine Liquid–liquid extraction Environmental Chemistry Sample preparation 030216 legal & forensic medicine Spectroscopy |
| Zdroj: | Drug Testing and Analysis. 9:808-823 |
| ISSN: | 1942-7603 |
| Popis: | Collection of oral fluid (OF) is easy and non-invasive compared to the collection of urine and blood, and interest in OF for drug screening and diagnostic purposes is increasing. A high-throughput ultra-high-performance liquid chromatography-tandem mass spectrometry method for determination of 21 drugs in OF using fully automated 96-well plate supported liquid extraction for sample preparation is presented. The method contains a selection of classic drugs of abuse, including amphetamines, cocaine, cannabis, opioids, and benzodiazepines. The method was fully validated for 200 μL OF/buffer mix using an Intercept OF sampling kit; validation included linearity, sensitivity, precision, accuracy, extraction recovery, matrix effects, stability, and carry-over. Inter-assay precision (RSD) and accuracy (relative error) were |
| Databáze: | OpenAIRE |
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