| Autor: |
Klas Norrby, G. Johannisson, J. Mellgren |
| Rok vydání: |
1967 |
| Předmět: |
|
| Zdroj: |
Experimental Cell Research. 48:582-594 |
| ISSN: |
0014-4827 |
| DOI: |
10.1016/0014-4827(67)90324-2 |
| Popis: |
428 complete cycles in HeLa cells were cinemicrographically recorded in four experiments. The net cell increase was exponential during the greater part of the recording, which took place when the cultures were approximately 21 to 91 hr old. Migration across the borders of the field of vision was random. Growth fraction was shown to be essentially 1.0. Whereas generation times as well as mitotic periods had positively skew distributions, the corresponding rates (reciprocals of times) were normally distributed. Mean generation times and rates differed significantly between the experiments. This variation of generation time was due to variation of the interphase. Positive correlation between generation times of sister cells was established, but none could be found between mother and daughter cells. Assuming that an exponential net cell increase in an asynchronous population constitutes a difference between exponential cell birth and exponential cell death, mathematically defined rates of birth, death and growth are presented. These mathematically calculated kinetic parameters were checked against comparable parameters derived from generation rates for individual cells and their distribution, as well as from the increase of cell mass in sister cultures. The weight of evidence indicates that our mathematically calculated birth and death rates adequately characterize the growth in vitro of an exponentially expanding cell population whose growth fraction is essentially unity. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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